Characterization of the interaction of galectin-3 with the natural cytotoxicity receptor NKp30

• Thesis title in Czech: Charakterizace interakce galektinu-3 s receptorem přirozené cytotoxicity NKp30
• Thesis title in English: Characterization of the interaction of galectin-3 with the natural cytotoxicity receptor NKp30
• Key words: Galektin-3, NKp30, NK buňky, HEK293T, N-glykosylace
• English key words: Galectin-3, NKp30, NK cells, HEK293T, N-glycosylation
• Academic year of topic announcement: 2022/2023
• Thesis type: diploma thesis
• Thesis language: angličtina
• Department: Department of Biochemistry (31-250)
• Supervisor: RNDr. Ondřej Vaněk, Ph.D.
• Author: hidden - assigned and confirmed by the Study Dept.
• Date of registration: 08.11.2022
• Date of assignment: 04.01.2023
• Confirmed by Study dept. on: 27.01.2023
• Date of electronic submission: 21.08.2024
• Date of proceeded defence: 10.09.2024
• Opponents: RNDr. Věra Černá, Ph.D.
• Advisors: Mgr. Celeste de Sousa Santos Abreu, Ph.D.
• Award: Thesis was put forward for an award

Preliminary scope of work

Náplní práce bude rekombinantní exprese rozpustné formy NK buněčného receptoru NKp30 a jeho nedávno popsaného proteinového ligandu galektinu 3 a studium jejich vzájemné interakce s cílem jejího strukturního popisu, ale i ověření role glykosylace receptoru NKp30 v této interakci. Rekombinantní exprese proteinů v bakteriálním expresním systému i v lidské buněčné linii HEK293, purifikace proteinů a jejich charakterizace pomocí základních i pokročilých biochemických a biofyzikálních technik, krystalizace proteinů.

Preliminary scope of work in English

The aim of the thesis is recombinant expression of soluble form of NK cell receptor NKp30 and its newly described protein ligand galectin 3 and study of their mutual interaction with respect to its structural description, but also with respect to evaluation of the role of NKp30 glycosylation within this interaction. Recombinant protein expression in both bacterial and human HEK293 cell line expression systems, protein purification and their characterization by basic and advanced biochemical and biophysical techniques, protein crystallization.