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Immune stimulation by lipid nanoparticles
| Thesis title in Czech: | Stimulace imunitní odpovědi lipidickými nanočásticemi |
|---|---|
| Thesis title in English: | Immune stimulation by lipid nanoparticles |
| Key words: | lipidické nanočástice, doprava mRNA, myší model, produkce protilátek, ionizovatelné lipidoidy |
| English key words: | lipid nanoparticles, mRNA delivery, mouse model, antibody production, ionizable lipidoids |
| Academic year of topic announcement: | 2020/2021 |
| Thesis type: | diploma thesis |
| Thesis language: | angličtina |
| Department: | Department of Genetics and Microbiology (31-140) |
| Supervisor: | Mgr. Klára Grantz Šašková, Ph.D. |
| Author: | hidden - assigned by the advisor, waiting for guarantor's approval |
| Date of registration: | 09.11.2020 |
| Date of assignment: | 09.11.2020 |
| Date of electronic submission: | 10.08.2022 |
| Date of proceeded defence: | 08.09.2022 |
| Opponents: | Mgr. Andrea Pačesová, Ph.D. |
| Preliminary scope of work |
| Studentka/student připraví a otestuje naše nově vyvinuté lipidické nanočástice obsahující mRNA z hlediska jejich schopnosti vyvolat imunitní odpověď. Naučí se připravovat a charakterizovat částice, navrhne cílové mRNA, naklonuje je a in vitro transkribuje ve vysokém výtěžku. Studentka či student se naučí, jak zacházet s tkáňovými kulturami různého původu, a v případě potřeby si osvojí znalosti, jak zacházet s experimenty na zvířatech (např. laboratorní myši). |
| Preliminary scope of work in English |
| The student will prepare and test our newly developed lipid nanopraticles harboring mRNA for their capabilty to induce immune response. She/he will learn how to prepare and characterize the particles, she/he will design the target mRNAs, clone them and in vitro trancribe them in high yield. The student will learn how to handle tissue cultures of various origin and if needed the student will also aquire knowledge how to handle animal (e.g. mice) experiments. |