The role of RNA-demethylase FTO in the energy metabolism of brain cells
| Thesis title in Czech: | Úloha RNA-demetylázy FTO v energetickém metabolizmu mozkových buněk |
|---|---|
| Thesis title in English: | The role of RNA-demethylase FTO in the energy metabolism of brain cells |
| Key words: | Alzheimerova choroba, kognitivní stárnutí, demence, astrocyt, neuron, metylace RNA |
| English key words: | Alzheimer's disease, cognitive aging, dementia, astrocyte, neuron, RNA methylation |
| Academic year of topic announcement: | 2017/2018 |
| Thesis type: | dissertation |
| Thesis language: | angličtina |
| Department: | Department of Physiology (31-152) |
| Supervisor: | Mgr. Petr Telenský, Ph.D. |
| Author: | hidden - assigned by the advisor |
| Date of registration: | 04.10.2017 |
| Date of assignment: | 04.10.2017 |
| Advisors: | doc. RNDr. Jiří Novotný, DSc. |
| Preliminary scope of work |
| 1. Stanovit vliv inhibice FTO na neuronální diferenciaci, oxidativní metabolismus, fosforylaci Tau, produkci ATP, oxidativní stress a viabilitu astrocytomových buněk koinkubovaných s peptidem Aβ 1-42 nebo streptozotocinem. 2. Stanovit vliv inhibice FTO na glykolytický metabolismus, aktivaci astrocytů a uptake glukózy v astrocytomových buňkách. |
| Preliminary scope of work in English |
| 1. Determine the effects of FTO-inhibition on neuronal differenciation, oxidative metabolism, Tau phosphorylation, ATP production, oxidative stress and viability of neuroblastoma cells co-incubated with Aβ 1-42 peptides or streptozotocin 2. Determine the effects of FTO-inhibition on glycolytic metabolism, astrocytic activation and glucose uptake in astrocytoma cells. |