Příprava nádorového ligandu B7-H6 s coiled-coil kotvou a ověření jeho vazby na receptor NKp30

• Thesis title in Czech: Příprava nádorového ligandu B7-H6 s coiled-coil kotvou a ověření jeho vazby na receptor NKp30
• Thesis title in English: Preparation of tumour ligand B7-H6 with coiled-coil tag and verifying of its binding to receptor NKp30
• Key words: NK buňky, rekombinantní exprese, B7-H6, HEK293, coiled-coil
• English key words: NK cells, recombinant expression, B7-H6, HEK293, coiled-coil
• Academic year of topic announcement: 2016/2017
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Biochemistry (31-250)
• Supervisor: RNDr. Ondřej Vaněk, Ph.D.
• Author: hidden - assigned by the advisor, waiting for guarantor's approval
• Date of registration: 11.11.2016
• Date of assignment: 12.11.2016
• Date of electronic submission: 14.08.2017
• Date of proceeded defence: 04.09.2017
• Opponents: RNDr. Michaela Čermáková, Ph.D.

Preliminary scope of work

Náplní práce bude rekombinantní exprese několika rozpustných domén lidských imunoreceptorů s coiled-coil kotvou, a to jak v bakteriálním, tak v savčím expresním systému. Coiled-coil kotva bude využita pro vazbu těchto domén na polymerní nosič, konečným cílem je sledování vlivu prezentace domén na polymerním nosiči na imunitní odpověď v modelovém systému nádorové linie s definovaným nádorovým antigenem při terapii protilátkou proti tomuto antigenu.

Tato práce je součástí projektu "Harnessing soluble forms of NK cell receptors and their ligands for the generation of novel anticancer immunotherapeutics" podpořeného prestižní cenou Instruct R&D Pilot Project Award z evropského programu Instruct a také projektem Grantové agentury ČR.

Preliminary scope of work in English

The aim of the thesis is recombinant expression of several soluble domains of human immunoreceptors with a coiled-coil tag, both in bacterial and in mammalian expression system. Coiled-coil tag will be utilized for attachment of these domains towards a polymeric carrier, the final goal is an evaluation of the influence of the domain presentation on polymeric carrier on the immune response in model system of tumor cell line with defined tumor antigen during the therapy by monoclonal antibody directed against this antigen.

This work is part of project "Harnessing soluble forms of NK cell receptors and their ligands for the generation of novel anticancer immunotherapeutics" supported by prestigious Instruct R&D Pilot Project Award from the European programe Instruct and also by project from Czech Science Foundation.