Pleiotropní účinky transkripčního faktoru Opi1 u Saccharomyces cerevisiae

• Thesis title in Czech: Pleiotropní účinky transkripčního faktoru Opi1 u Saccharomyces cerevisiae
• Thesis title in English: Pleiotropic effects of transcriptional protein Opi1 in Saccharomyces cerevisiae
• Key words: biosyntéza fosfolipidů, inositol, cholin, regulace genové exprese, OPI1, INO2, INO4
• English key words: phospholipid biosynthesis, inositol, choline, gene expression regulation, OPI1, INO2, INO4
• Academic year of topic announcement: 2013/2014
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Genetics and Microbiology (31-140)
• Supervisor: RNDr. Michaela Schierová, Ph.D.
• Author: hidden - assigned by the advisor, waiting for guarantor's approval
• Date of registration: 11.11.2013
• Date of assignment: 25.11.2013
• Date of electronic submission: 15.05.2014
• Date of proceeded defence: 05.06.2014
• Opponents: RNDr. Tomáš Mašek, Ph.D.

Preliminary scope of work

Stručný přehled metabolismu fosfolipidů.
Mechanismy regulace metabolismu fosfolipidů.
Struktura a funkce proteinu Opi1.
Fenotypové projevy mutací OPI1.
Změny v expresi regulačních i strukturních genů metabolismu fosfolipidů v závislosti na růstové fázi a dostupnosti inositolu.

Preliminary scope of work in English

A brief overview of the phospholipid metabolism.
Mechanisms of regulation of phospholipid metabolism.
Structure and function of the Opi1 protein.
OPI1 mutations phenotype.
Expression changes of phospholipid metabolism regulatory and structural genes depending on the growth phase and the availability of inositol.