Study of structure and interaction of human lymphocyte receptors

• Thesis title in Czech: Studium struktury a interakcí lidských lymfocytárních receptorů
• Thesis title in English: Study of structure and interaction of human lymphocyte receptors
• Key words: receptor, LLT1, NKR-P1, NK buňka, HEK293
• English key words: receptor, LLT1, NKR-P1, NK cell, HEK293
• Academic year of topic announcement: 2012/2013
• Thesis type: dissertation
• Thesis language: angličtina
• Department: Department of Biochemistry (31-250)
• Supervisor: RNDr. Ondřej Vaněk, Ph.D.
• Author: hidden - assigned and confirmed by the Study Dept.
• Date of registration: 11.10.2012
• Date of assignment: 14.11.2012
• Confirmed by Study dept. on: 14.11.2012
• Date and time of defence: 22.09.2017 10:00
• Date of electronic submission: 04.08.2017
• Date of proceeded defence: 22.09.2017
• Opponents: doc. RNDr. Miroslav Šulc, Ph.D.

Preliminary scope of work

S použitím eyukaryotického expresního systému HEK293 buněk budou produkovány rozpustné formy lidských lymfocytárních receptorů, a to především vazebných partnerů NKR-P1 a LLT-1, NKp65 a KACL, NKp80 a AICL, a jejich interakce budou studovány metodami biochemie, strukturní biologie a imunologie.

Preliminary scope of work in English

Using eukaryotic expression in HEK293 cells, soluble forms of human lymphocyte receptors will be produced, especially of the binding partners NKR-P1 and LLT1, NKp65 and KACL, NKp80 and AICL, and their interaction will be studied using techniques of biochemistry, structural biology and immunology.