Úloha Hac1p pri morfogenéze kvasinkových kolónií

• Thesis title in thesis language (Slovak): Úloha Hac1p pri morfogenéze kvasinkových kolónií
• Thesis title in Czech: Úloha Hac1p při morfogenezi kvasinkových kolonií
• Thesis title in English: The effect of HAc1p on the development of yeast colony
• Key words: kvasinky, strukturované kolonie, Hac1p, stres v ER, Flo11p, Sec61p
• English key words: yeast, structured colony, Hac1p, unfolded protein response, Flo11p, Sec61p
• Academic year of topic announcement: 2011/2012
• Thesis type: diploma thesis
• Thesis language: slovenština
• Department: Department of Genetics and Microbiology (31-140)
• Supervisor: RNDr. Michaela Schierová, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 24.11.2011
• Date of assignment: 05.12.2011
• Date of electronic submission: 15.08.2013
• Date of proceeded defence: 17.09.2013
• Opponents: Ing. Iva Pichová, CSc.

Preliminary scope of work

Popísať funkciu proteínu Hac1p pri vývoji kolónií u Saccharomyces cerevisiae.
Sledovanie vplyvu delécie génu HAC1 u prírodných kmeňov kvasiniek Saccharomyces cerevisiae odlišného pôvodu.
Príprava kmeňov so zvýšenou expresiou HAC1.
Rastová analýza kmeňov za neselektívnych i selektívnych podmienok. Testovanie citlivosti k inhibítorom.
Detekcia produkcie vybraných proteínov imunodetekciou a pomocou fluorescencie.

Preliminary scope of work in English

Review of Hac1p function in the colony development of Saccharomyces cerevisiae.
Study of HAC1 deletion effect on colony morphology of wild type strains in Saccharomyces cerevisiae.
The development of their colonies under control and selective conditions.
Growth curves. Inhibitory tests.
Study of selected proteins by immunodetection and fluorescence microscopy.