Cellular functions of archamebae

• Název práce v češtině: Buněčné funkce archaméb
• Název v anglickém jazyce: Cellular functions of archamebae
• Klíčová slova: peroxiny, entamoeba, mastigamoeba
• Klíčová slova anglicky: peroxins, entamoeba, mastigamoeba
• Akademický rok vypsání: 2016/2017
• Typ práce: disertační práce
• Jazyk práce: angličtina
• Ústav: Katedra parazitologie (31-161)
• Vedoucí / školitel: prof. RNDr. Jan Tachezy, Ph.D.
• Řešitel: skrytý - zadáno vedoucím/školitelem
• Datum přihlášení: 20.10.2016
• Datum zadání: 20.10.2016

Předběžná náplň práce v anglickém jazyce

Peroxisomes are a single membrane bound organelles 0,1-1 µm in diameter that evolved in eukaryotic cells most likely to compartmentalize pathways generating toxic products and thus protect the cell from the damage. It is generally believed that peroxisomes are absent in parasitic protists living under anaerobiosis or oxygen-poor environments such as urogenital pathogen Trichomonas vaginalis, and intestinal parasites Giardia intestinalis and Archamoebae (e.g. Entamoeba histolytica). Unexpectedly, genome analysis of Mastigamoeba balamuthi, a free-living member of Archamoebae group, revealed presence of 13 genes coding for proteins with homology to peroxins. The set of putative peroxins includes Pex3, and Pex19, which are essential for peroxisome biogenesis. This finding strongly suggests that Mastigamoeba balamuthi possesses peroxisomes and that these organelles operate under anaerobiosis. Moreover, searches for peroxins in the genome of E. histolytica using M. balamuthi genes as queries revealed 6 putative peroxins also in E. histolytica genome. Although presence of peroxisome in this organism based on such a limited set of peroxins is less probable than in M. balamuthi, it is of great interest to investigate cellular localization and possible function of these proteins.